BME140, Fall 2011, Section 01: Syllabus

TuTh 4:00 pm - 5:45 pm, Baskin Engineering 287

Course Description

This course will explore both theoretical and practical concepts of bioinstrumentation and its application in a variety of medical and scientific disciplines. This course highlights the basic laboratory skills that are essential for beginning-level employment in clinical, pharmaceutical and biotechnology laboratories. Analytical experiments will be performed in the laboratory portion of the class. Topics covered include plasmid purification from bacteria, transformation of plasmid DNA to bacteria, Polymerase Chain Reaction (PCR), DNA sequencing with Pyrosequencing, and gel-electrophoresis of biomolecules. There will also be a unit on principles of biosensors, including in-depth study of the nanopipette as a biosensor platform.

This course is well-suited for students in all majors and programs, and involves a lab demo as well as literature workshop of selected articles. Critical evaluation of literature will be performed in groups. Each group presents one article and will critique two other groups' articles. This project aims to teach critical reading, interpretation and comparison of the most advanced techniques and platforms in the fields of genomics proteomics and related instrumentations. The project requires teamwork, planning, and participation. Student presence on the laboratory demos as well workshop days is compulsory.


Homework: 10%

Participation: 10%

Attendance: 10%

Lab Reports: 25%

Oral Presentation: 20%

Final Exam: 25%


To receive credit for attendance on a given day, students must sign an attendance sheet during each class period.

Late Submissions

Late lab reports and homework will be docked one full letter grade per day past the deadline.

Final Exam

7:30 - 10:30 pm, Tuesday, December 6th

The final exam is cumulative. All topics covered in the course may appear on the final.

Academic Integrity

Students are expected to follow the guidelines set by UCSC for academic integrity. While students are expected to work in groups, the written work submitted by each student must be their own. More information can be found at the Official University Policy on Academic Integrity for Undergraduate Students.