Molecular Biomechanics Laboratory

BME150L/250L: Molecular Biomechanics Lab

Syllabus Spring 2014


Laboratory Instructors         Hugh Olsen/Jeff Nivala/Logan Mulroney

Email:                /

Office hours:             By appointment

Meeting Times/Location

Location:             Baskin Engineering 287

Section 1:            Tues 5-8 PM

Section 2:            Wed 5-8 PM


     Readings and resources will be posted on the course webpage

     You will keep your lab notebook for the class on the class web site

     Provided For You- Lab coats, safety glasses, and gloves


Course Goal Experience cloning, expressing, and purifying recombinant proteins.

Course Structure

     You will be working in pairs

     For certain labs you will need to come in early or the next day for the experiments.

Student Evaluation

Attendance:                                     10%

Lab notebook/write-up:            50%                                               

Practical:                                    15%

Lab Final                                    25%


Lab notebooks will be maintained on the class web site and each student will have their own notebook. Your notebook will be graded on the following:

(1)Pre-lab questions

(2)Statement of the plan for the session and the purpose of the experiment

(3)Log of activities, regents and materials used

(4)Results, what you learned from the activity

(5)Question(s) of the day (this will be presented in class).



 The lab final will consist of two parts, a practicum and a written exam. The lab practicum will evaluate how well you use laboratory equipment and procedures.

The written test, evaluates your understanding of the techniques and theory taught in lab. Lab final will be the last day of laboratory instruction (see schedule below for date).


Students are expected to attend each lab session.  This will be 10% of your grade. Each session you will use samples that you generated from previous sessions.  If you miss a session, contact Hugh or Jeff to attend one of the other sections to make up the work.

Lab Safety

No food is allowed in lab.  Please wear appropriate attire (long pants, closed toe shoes). Lab coats, safety glasses, and gloves will be provided. Drink and food can be set out in the hall way on the table next to the laboratory door.

Disability Resource Center

If you qualify for classroom accommodations because of a disability, please submit your Accommodation Authorization from the Disability Resource Center (DRC) to one of the instructors in a timely manner, preferably within the first two weeks of the quarter. Contact the DRC at 459-2089 (voice), 459-4806 (TTY).

Academic Misconduct

By enrolling in the University, students are automatically agreeing to abide by University policies, including those on academic misconduct. Academic integrity and scholarship are core values that should guide our conduct and decisions as members of the UCSC community. Plagiarism and cheating contradict these values, and so are very serious academic offenses. Penalties can include a failing grade in an assignment or in the course, or suspension or expulsion from the university. Students are expected to familiarize themselves with and follow citation practices. The instructors in this course will pursue disciplinary action in all instances of academic misconduct.


Instructors and Assistants